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Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

13.7K Views

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06:58 min

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June 1st, 2015

DOI :

10.3791/52853-v

June 1st, 2015

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Alzahra J. Al Omran¹, Hannah C. Saternos¹, Tongyu Liu², Surya M. Nauli³, Wissam A. AbouAlaiwi¹

¹Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, ²Life Sciences Institute, University of Michigan, ³Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus

Summary

Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties.

Explore More Videos

Ependymal Cilia

Lateral Ventricles

Multiciliated Ependymal Cells

Ciliary Dynamics

Ciliary Beating Frequency

Calcium Oscillations

Immunofluorescence Imaging

Neurological Deficits

Chapters in this video

0:05

Title

1:31

Brain Extraction, Sectioning, and Tissue Preparation

2:28

Live Imaging Configuration and Setup

4:00

Calcium Signal Recording and Immunofluorescence Microscopy

5:26

Results: Measuring Ependymal Cilia Function and Calcium Signaling

6:22

Conclusion

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