Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

8.5K Views

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10:55 min

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December 16th, 2017

DOI :

10.3791/56291-v

December 16th, 2017

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Rosa Gómez-Villafuertes*¹^,²^,³, Lucía Paniagua-Herranz*¹^,²^,³, Sergio Gascon*⁴^,⁵, David de Agustín-Durán¹^,²^,³, María de la O Ferreras¹^,²^,³, Juan Carlos Gil-Redondo¹^,²^,³, María José Queipo¹^,²^,³, Aida Menendez-Mendez¹^,²^,³, Ráquel Pérez-Sen¹^,²^,³, Esmerilda G. Delicado¹^,²^,³, Javier Gualix¹^,²^,³, Marcos R. Costa⁶, Timm Schroeder⁷, María Teresa Miras-Portugal¹^,²^,³, Felipe Ortega¹^,²^,³

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, ⁶Brain Institute, Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich

Transcript

Summary

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

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