JoVE Journal

Developmental Biology

This content is Open Access.

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.

Chapters in this video

0:05

Title

1:14

Setting of the Triaxial Coils

2:01

Preparation of Plant Materials and Conditions for Plant Growth

5:10

Quantitative Real Time-PCR (qPCR) Analysis of Arabidopsis Gene Expression

8:05

Results: Effects of Geomagnetic Field Reversal on Arabidopsis Morphology, Gene Expression, and Antioxidant-related Gene Expression

9:23

Conclusion

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