JoVE Journal

Developmental Biology

This content is Open Access.

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Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Transcript

We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.

Chapters in this video

0:05

Title

0:44

3D "Organoid" Differentiation

4:32

Alternative 2D Differentiation Culture

8:28

Results: 3D Products Exhibit Markers and Structures of Early Neuroepithelium, and 2D Products Exhibit Cerebellar Markers and Functional Neuronal Activity

9:48

Conclusion

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