Uppsala Biomedical Center, Uppsala University

1 ARTICLES PUBLISHED IN JoVE

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Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Farzaneh Assadian¹, Karl Sandström², Göran Laurell², Catharina Svensson¹, Göran Akusjärvi¹, Tanel Punga¹

¹Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, ²Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset

Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.