The New York State Office for People with Developmental Disabilities

2 ARTICLES PUBLISHED IN JoVE

Chemistry

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Mohameedyaseen Syedbasha¹, Janina Linnik^1,2,3, Deanna Santer⁴, Daire O'Shea⁵, Khaled Barakat^4,6, Michael Joyce⁴, Nina Khanna⁷, D. Lorne Tyrrell⁴, Michael Houghton⁴, Adrian Egli^1,8

¹Applied Microbiology Research, Department of Biomedicine, University of Basel, ²Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, ³Swiss Institute of Bioinformatics, ⁴Li Ka Shing Institute for Virology, University of Alberta, ⁵Regional Infectious Diseases Unit, University of Edinburgh, ⁶Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, ⁷Infection Biology, Department of Biomedicine, University of Basel, ⁸Clinical Microbiology, University Hospital Basel

The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

Developmental Biology

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

George Merz¹, Valerie Schwenk¹, Ruchit Shah², Carolyn Salafia^1,2, Phillip Necaise², Michael Joyce¹, Tom Villani³, Michael Johnson³, Nick Crider³

¹The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, ²Placental Analytics LLC, ³Visikol Inc.

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm³.