To begin, use the rotary tool and the appropriate carving bit to drill two irrigation holes in the top of the lid. On the side, drill a one-centimeter opening. Use the electric rotary tool to drill a notch in the side of the Petri dish bottom for the plant stem.
Next, using the electric rotary tool, equipped with the appropriate drill bits, drill a 30-millimeter rectangular notch down to the bottom of the Petri dish. For high-resolution observation, drill circular holes approximately 25 millimeters in diameter in the bottom of the Petri dish. Ensure the holes are not drilled directly under the lid's irrigation holes.
Using the metal guide and paperclip, wedge the piece of the nylon mesh filter membrane in place and press it firmly onto the plastic barrier on the root or notch side. Then, align the top of the membrane with the top of the barrier. Using the pyrography kit, cut along the edge of the metal guide to melt and seal the membrane to the bottom of the Petri dish and the plastic barrier separating the two compartments.
With the metal guide and paperclips still in place, use tweezers to carefully remove excess nylon mesh filter membrane. Under the dissecting microscope, verify that the nylon mesh filter membrane is properly sealed to the plastic to prevent root penetration into the HIFULL compartment. Then, apply the silicone sealant along the edge of the circular holes on the outside of the Petri dish bottom.
Place the cover slip on the outside of the Petri dish bottom and gently press for perfect adhesion.
