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Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

12.6K Views

09:39 min

January 27th, 2014

DOI :

10.3791/51218-v

January 27th, 2014


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Adenylyl Cyclase

Capítulos en este video

0:05

Title

1:37

Expansion and Cryopreservation of Assay Ready Cells

2:58

Plating Assay Ready Cells

4:15

Small Molecule Screening and Persistent Agonist Treatment

5:16

Stimulation, Quenching, and Estimation of cAMP Accumulation

7:02

Results: Development and Testing of a Cell-based 384 Well Sensitization Assay with Receptor Antagonists

9:12

Conclusion

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