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JoVE Journal
Bioengineering
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Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
12.6K Views
•
09:39 min
•
January 27th, 2014
DOI :
January 27th, 2014
•Chapters in this video
0:05
Title
1:37
Expansion and Cryopreservation of Assay Ready Cells
2:58
Plating Assay Ready Cells
4:15
Small Molecule Screening and Persistent Agonist Treatment
5:16
Stimulation, Quenching, and Estimation of cAMP Accumulation
7:02
Results: Development and Testing of a Cell-based 384 Well Sensitization Assay with Receptor Antagonists
9:12
Conclusion
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ISSN 2578-2614
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