Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

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10.6K Views

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11:51 min

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August 20th, 2009

DOI :

10.3791/1510-v

August 20th, 2009

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Claudio Vinegoni¹, Daniel Razansky², Chrysoula Pitsouli³, Norbert Perrimon³, Vasilis Ntziachristos², Ralph Weissleder¹

¹Center for Systems Biology, Massachusetts General Hospital, ²Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, ³Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

Résumé

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

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