The overall goal of this procedure is to isolate, expand and differentiate mesenchymal stems sells derived from canine ovarian tissue. This experiment was performed with the ovaries of four mongrel female dogs donated after elective surgery at a canine sterilization program. This experiment was approved by the Ethics Committee on the use of animals of Sao Paulo State University, FVAC.
Representative results.Discussion.Conclusion.
Herein, we describe a method for the isolation, expansion, and differentiation of mesenchymal stem cells from canine ovarian tissue.