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Developmental Biology

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Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

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5.6K Views

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11:41 min

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December 16th, 2018

DOI :

10.3791/58163-v

December 16th, 2018

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Amanda B.T. Hill¹^,², Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), ²CRRF, Université de Montréal, ³FZEA, University of São Paulo, ⁴FMVZ, University of São Paulo

Transcrição

The overall goal of this procedure is to isolate, expand and differentiate mesenchymal stems sells derived from canine ovarian tissue. This experiment was performed with the ovaries of four mongrel female dogs donated after elective surgery at a canine sterilization program. This experiment was approved by the Ethics Committee on the use of animals of Sao Paulo State University, FVAC.

Representative results.Discussion.Conclusion.

Resumo

Herein, we describe a method for the isolation, expansion, and differentiation of mesenchymal stem cells from canine ovarian tissue.

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