National Taiwan Ocean University

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Hsien-San Hou¹, Hui-Fang Chang¹, Ji-Yen Cheng^1,2,3,4,5

¹Research Center for Applied Sciences, Academia Sinica, ²Institute of Biophotonics, National Yang-Ming University, ³Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, ⁴Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, ⁵Ph.D. Program in Microbial Genomics, National Chung Hsing University

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.

Bioengineering

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Tzu-Yuan Chou¹, Yung-Shin Sun², Hsien-San Hou³, Shang-Ying Wu¹, Yun Zhu¹, Ji-Yen Cheng³, Kai-Yin Lo¹

¹Department of Agricultural Chemistry, National Taiwan University, ²Department of Physics, Fu-Jen Catholic University, ³Research Center for Applied Sciences, Academia Sinica

Micro-fabricated devices integrated with fluidic components provide an in vitro platform for cell studies mimicking the in vivo micro-environment. We developed polymethylmethacrylate-based microfluidic chips for studying cellular responses under single or coexisting chemical/electrical/shear stress stimuli.

Engineering

A High Performance Impedance-based Platform for Evaporation Rate Detection

Wei-Lung Chou¹, Pee-Yew Lee², Cheng-You Chen², Yu-Hsin Lin³, Yung-Sheng Lin⁴

¹Department of Safety, Health and Environmental Engineering, Hungkuang University, ²Institute of Materials Engineering, National Taiwan Ocean University, ³Instrument Technology Research Center, National Applied Research Laboratories, ⁴Department of Chemical Engineering, National United University

This paper presents an impedance-based apparatus for evaporation rate detection of solutions. It offers clear advantages over a conventional weight loss approach: a fast response, high-sensitivity detection, a small sample requirement, multiple sample measurements, and easy disassembly for cleaning and reuse purposes.

Chemistry

Synthesizing Sodium Tungstate and Sodium Molybdate Microcapsules via Bacterial Mineral Excretion

Pao-Hung Lin¹, Ying-Tang Huang², Fu-Wen Lin³

¹Department of Electronic and Computer Engineering, National Taiwan University of Science and Technology, ²Department of Marine Biotechnology, National Kaohsiung Marine University, ³Institute of Applied English, National Taiwan Ocean University

This work presents a protocol for manufacture sodium tungstate and sodium molybdate microcapsules via bacteria and their corresponding nanoparticles.

Bioengineering

Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices

Hui-Fang Chang¹, Shih-En Chou¹, Ji-Yen Cheng^1,2,3,4

¹Research Center for Applied Sciences, Academia Sinica, ²Institute of Biophotonics, National Yang Ming Chao Tung University, ³Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, ⁴College of Engineering, Chang Gung University

In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system.

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