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Humanitas Clinical and Research Center, Rozzano, Milan, Italy
2 ARTICLES PUBLISHED IN JoVE
Genetics
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Oriana Romano 1,2, Ingrid Cifola 3, Valentina Poletti 4, Marco Severgnini 3, Clelia Peano 3, Gianluca De Bellis 3, Fulvio Mavilio 1,4, Annarita Miccio 1,2,4,5
1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes
Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.
Biology
Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Maria Felicia Soluri 1, Simone Puccio 2, Giada Caredda 2, Giorgio Grillo 3, Vito Flavio Licciulli 3, Arianna Consiglio 3, Paolo Edomi 4, Claudio Santoro 1, Daniele Sblattero 4, Clelia Peano 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy
The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.
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