JoVE Journal

Immunology and Infection

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Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

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要約

Structure-based drug design plays an important role in drug development. Pursuing multiple targets in parallel greatly increases the chance of success for lead discovery. The following article highlights how the Seattle Structural Genomics Center for Infectious Disease utilizes a multi-target approach for gene-to-structure determination of the PB2 influenza A subunit.

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Parallel Processing

Gene to structure Determination

Influenza Polymerase PB2 Subunit

Pandemic Outbreaks

Influenza Virus Infection

Point Mutations

Virulence Factor

Antiviral Drug Target

Structural Genomics Program

National Institute Of Allergy And Infectious Disease NIAID

Seattle Structural Genomics Center For Infectious Disease SSGCID

Three dimensional Protein Structures

NIAID Category A C Pathogens

Structure based Drug Design

Drug Development

この動画の章

0:05

Title

2:20

Construct Design

4:07

Polymerase Incomplete Primer Extension (PIPE) Cloning

6:14

Expression Optimization

8:21

Large Scale Fermentation

9:40

Protein Purification - Cell Lysis

10:54

Protein Purification - Affinity Chromatography

13:04

Protein Purification - Affinity Tag Cleavage

14:47

Protein Purification - Size-exclusion Chromatography (SEC)

15:42

Protein Crystallization

17:03

Crystal Harvesting

18:15

Crystal Screening/Data Collection

19:27

Results: 3D Structure Determination of Influenza Virus PB2

21:05

Conclusion

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