Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
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22:10 min
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June 28th, 2013
DOI :
June 28th, 2013
•Brianna L. Armour*1, Steve R. Barnes*2, Spencer O. Moen2, Eric Smith3, Amy C. Raymond4, James W. Fairman5, Lance J. Stewart6, Bart L. Staker7, Darren W. Begley5, Thomas E. Edwards8, Donald D. Lorimer9
1Protein Crystallization Lab, Emerald Bio, 2Molecular Biology Lab, Emerald Bio, 3Scientific Sales Representative, Emerald Bio, 4Group Leader II, Emerald Bio, 5Group Leader I, Emerald Bio, 6Chair of Advisory Board, Emerald Bio, 7Director of Multi-Target Services, Emerald Bio, 8Senior Project Leader, Emerald Bio, 9Project Leader II & SSGCID Site Manager, Emerald Bio
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0:05
Title
2:20
Construct Design
4:07
Polymerase Incomplete Primer Extension (PIPE) Cloning
6:14
Expression Optimization
8:21
Large Scale Fermentation
9:40
Protein Purification - Cell Lysis
10:54
Protein Purification - Affinity Chromatography
13:04
Protein Purification - Affinity Tag Cleavage
14:47
Protein Purification - Size-exclusion Chromatography (SEC)
15:42
Protein Crystallization
17:03
Crystal Harvesting
18:15
Crystal Screening/Data Collection
19:27
Results: 3D Structure Determination of Influenza Virus PB2
21:05
Conclusion
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