Primer-Free Aptamer Selection Using A Random DNA Library
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11:14 min
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July 26th, 2010
DOI :
July 26th, 2010
•1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University
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0:04
Title
0:12
Introduction
1:39
Preparation of PF Library-DNA Fragments
3:26
Preparation of S100B-bound Beads
4:55
Selection & Recovery of Aptamers
6:40
TOPO Cloning and Sequencing
7:17
Sandwich Binding Assays
9:11
Representative Results
10:35
Conclusion
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