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JoVE Journal
Biology
This content is Open Access.
Primer-Free Aptamer Selection Using A Random DNA Library
24.6K Views
•
11:14 min
•
July 26th, 2010
DOI :
July 26th, 2010
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Chapters in this video
0:04
Title
0:12
Introduction
1:39
Preparation of PF Library-DNA Fragments
3:26
Preparation of S100B-bound Beads
4:55
Selection & Recovery of Aptamers
6:40
TOPO Cloning and Sequencing
7:17
Sandwich Binding Assays
9:11
Representative Results
10:35
Conclusion
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