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A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

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18.5K Views

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06:43 min

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August 31st, 2013

DOI :

10.3791/50512-v

August 31st, 2013

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Karen F. Underwood¹, Maria T. Mochin¹, Jessica L. Brusgard², Moran Choe³, Avi Gnatt⁴, Antonino Passaniti³^,⁵

¹Greenebaum Cancer Center, University of Maryland School of Medicine, ²Program in Molecular Medicine, University of Maryland School of Medicine, ³Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, ⁴Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, ⁵Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Смотреть дополнительные видео

Protein DNA Interactions

Transcriptional Regulators

Protein DNA Interaction

Quantitative Assay

Drug Screening

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0:05

Title

1:49

Cell Culture and Nuclear Protein Isolation

2:46

Preparation of 96-well Plates by Incubation with Nuclear Extracts

3:58

Measurement of RUNX2 DNA Binding

5:36

Results: Inhibition of RUNX2 DNA Binding

6:22

Conclusion

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