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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
21.6K Views
•
15:42 min
•
March 6th, 2009
DOI :
March 6th, 2009
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Chapters in this video
0:00
Title
0:09
Introduction
1:05
T Cell Staining
3:21
Reagents and Equipment
5:44
Assemble Materials and Equipment at Workspace
6:04
Step 1: PBMC Isolation
8:51
Step 2: CD4+ Cell Separatioin
11:01
Step 3: In Vitro Expansion Culture
13:34
Step 4: Visualizing T Cells by Tetramer Staining
15:33
Conclusion
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