Sign In
Methods Article
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
37.8K Views
•
15:21 min
•
August 7th, 2009
DOI :
August 7th, 2009
•Explore More Videos
Chapters in this video
0:05
Title
0:41
Introduction
1:03
Extraction of Total RNA using TRIzol Reagent
3:55
RNA Cleanup using the Qiagen RNEasy Mini Kit
7:57
cDNA Synthesis using the Invitrogen SuperScript First-Strand System
10:28
cDNA Isolation and Precipitation
13:50
Representative Results/Outcome
14:57
Conclusion
Related Videos
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
25.7K Views
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
11.6K Views
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
24.0K Views
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
15.4K Views
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
36.4K Views
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
18.0K Views
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
27.0K Views
Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
11.5K Views
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
14.7K Views
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
12.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.