JoVE Journal

Neuroscience

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Laser Capture Microdissection of Drosophila Peripheral Neurons

In this video-article we present a method for isolating single or multiple Drosophila da neurons from third instar larvae using the infrared capture (IR) class of Laser Capture Microdissection (LCM). RNA obtained from the isolated neurons can be readily used for downstream applications including qRT-PCR or microarray analyses.

Chapters in this video

0:00

Title

1:18

Introduction

1:53

Preparing the Larvae

3:29

Dehydration and Cuticle Removal from Frozen Larval Sections

5:13

Laser Capture Microdissection

8:11

RNA Isolation from LCM Derived Cells

9:29

Representative Results

11:00

Conclusion

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