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Neuroscience

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Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method

17.2K Views

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09:19 min

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September 6th, 2010

DOI :

September 6th, 2010

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Han-peng Xu¹^,², Lin Gou³^,⁴, Hong-Wei Dong³

¹Department of Neurosurgery, Cedars Sinai Medical Center, UCLA, ²Basic Medicine School, Fourth Military Medical University, ³Department of Neurology, David Geffen School of Medicine, UCLA, ⁴Aerospace Medicine School, Fourth Military Medical Univeristy

Summary

In this protocol, we described a new method to study the influence of glial cell heterogeneity on axon growth with an in vitro co-culture system. Rat cortical glial cells were cultured to confluence and cocultured with highly purified rat dorsal root ganglia neurons. Different glial cell influence on neurons adhesion and axon growth was compared directly in the same culture. This method provides a new way to directly study the glial cell heterogeneity influence on neuron adhesion and axon growth.

Explore More Videos

Glial Cell Heterogeneity

Coculture Method

Central Nervous System

Regenerative Capacity

Inhibitory Myelin

Neuron Behavior

Dorsal Root Ganglia Neurons

Chapters in this video

0:00

Title

1:03

Introduction

1:34

Establishing a Glial Cell Culture

4:29

Isolation, Purification, and Cuturing of Dorsal Root Ganglion Neurons (DRGs) and Coculturing of DRGs with Glial Cells

7:29

Representative Cultures of Glial Cells, DRG Neurons, and Cocultures of Both Cell Types

8:36

Conclusion

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