Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

12.6K Views

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09:28 min

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August 7th, 2010

DOI :

10.3791/2203-v

August 7th, 2010

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Raja S. Vasireddy¹^,²^,³, Michelle M. Tang¹^,², Li-Jeen Mah²^,³, George T. Georgiadis², Assam El-Osta¹^,³, Tom C. Karagiannis²^,³

¹Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, ²Epigenomic Medicine, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, ³Department of Pathology, University of Melbourne

Summary

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Spatial Distribution

H2AX

Ionizing Radiation

DNA Double strand Breaks DSBs

Ser 139 Residue

Histone H2AX

Phosphorylation

Phosphatidyl inosito 3 kinase PI3K Family

Ataxia Telangiectasia Mutated ATM

DNA protein Kinase Catalytic Subunit

ATM And RAD3 related ATR

Immunofluorescence Microscopy

H2AX Foci

DSB Formation And Repair

Radiation Modifying Compounds

Cytotoxic Compounds

DNA Damage And Repair

Chromatin Template

irradiation induced H2AX Foci

Chapters in this video

0:00

Title

0:05

Introduction

1:01

Cell Preparation

2:28

Immunofluorescence Staining

5:00

Microscopy

6:12

Analysis Foci Counting

7:56

Analysis Line Scan

8:39

Analysis 3D-Image Reconstruction

9:09

Conclusion

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