JoVE Journal
Biology
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Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
14.6K Views
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08:53 min
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February 17th, 2011
DOI :
February 17th, 2011
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Chapters in this video
0:05
Title
2:01
Preparation of Cell Line with an Integrated Recombination Substrate
3:04
Transfection to Deplete Endogenous BRCA1 and add back Mutant BRCA1
5:19
Analysis of Transfectants
6:07
Analysis of the Effects of BRCA1 Mutations on Homologous Recombination via GFP-Expression
7:54
Conclusion
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