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Medicine

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Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

17.4K Views

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11:22 min

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May 21st, 2013

DOI :

May 21st, 2013

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Markus T. Berninger¹^,², Gabriele Wexel³, Ernst J. Rummeny², Andreas B. Imhoff¹, Martina Anton³, Tobias D. Henning*²^,⁴, Stephan Vogt*¹

¹Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar der Technischen Universität München, ²Department of Radiology, Klinikum rechts der Isar der Technischen Universität München, ³Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, ⁴Department of Radiology, Uniklinik Köln

Summary

An experimental technique for the treatment of osteochondral defects in the rabbit's knee joint is described. The implantation of allogeneic mesenchymal stem cells into osteochondral defects provides a promising development in the field of tissue engineering. The preparation of fibrin-cell-clots in vitro offers a standardized method for implantation.

Explore More Videos

Osteochondral Defects

Rabbit s Knee Joint

Allogeneic Mesenchymal Stem Cells

Articular Cartilage

Subchondral Bone

Surgical Treatment Options

Autologous Transplants

Allogeneic Transplants

Donor Site Morbidity

Chapters in this video

0:05

Title

1:36

Preparation of a Donor Rabbit for the Isolation of Mesenchymal Stem Cells

3:00

Flushing of Rabbit MSC from Bones and Expansion

6:09

Preparation of Fibrin Clots in vitro

7:57

Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

9:32

Results: Successful Isolation and Implantation of Allogeneic Mesenchymal Stem Cells into an Artificial Osteochondral Defect

10:42

Conclusion

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