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Biology

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Methods Article

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

13.3K Views

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11:33 min

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March 12th, 2013

DOI :

10.3791/50145-v

March 12th, 2013

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Clemens Köhncke¹^,², Ulrike Lisewski¹, Leonhard Schleußner¹, Carolin Gaertner¹, Saskia Reichert¹, Torsten K. Roepke¹^,³

¹Experimental and Clinical Research Center (ECRC), Charité Medical Faculty and Max-Delbrück Center for Molecular Medicine (MDC), ²Medical Department, Division of Cardiology, Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, ³Medical Department, Division of Cardiology and Angiology, Campus Mitte, Charité - Universitätsmedizin Berlin

Summary

Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.

Explore More Videos

Kv Channel Ancillary Subunits

Cardiac Arrhythmias

Long QT Syndrome

Atrial Fibrillation

Electrophysiology

Langendorff Apparatus

Chapters in this video

0:05

Title

1:37

Animal Anesthesia and Organ Harvesting

3:00

Preparation of Heart and Langendorff Perfusion

4:03

Dissociation of Cardiomyocytes

6:30

Preparation of Cardiomyocytes for Cellular Electrophysiology, Biochemical of IF Studies

8:53

Cellular Electrophysiology

10:33

Results: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

11:04

Conclusion

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