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JoVE Journal
Biology
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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
20.5K Views
•
16:07 min
•
March 30th, 2013
DOI :
March 30th, 2013
•Chapters in this video
0:05
Title
2:06
Stamp Frozen Library Plates onto Rectangular Amp/Tet LB Agar Plates
3:16
Grow and Seed RNAi Bacterial Clones and Add Worms to RNAi Plates
5:49
Fix and Stain Worms in Nile Red and Image on a High-throughput Scope
7:22
Wash Worms and Image on a High-throughput Microscope
8:31
Biochemical Lipid Determination Using Solid Phase Extraction and Gas Chromatography-mass Spectrometry
12:02
Representative Fat Mass Content Results
15:39
Conclusion
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