JoVE Journal

Bioengineering

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Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

Chapters in this video

0:05

Title

1:18

Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)

3:16

Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells

4:19

In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice

6:50

Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs

8:43

Conclusion

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