JoVE Journal

Bioengineering

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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Induced pluripotent stem cell (iPSC)-derived myogenic progenitors are promising candidates for cell therapy strategies to treat muscular dystrophies. This protocol describes transplantation and functional measurements required to evaluate the engraftment and differentiation of iPSC-derived mesoangioblasts (a type of muscle progenitors) in mouse models of acute and chronic muscle regeneration.

Chapters in this video

0:05

Title

1:48

Assessment of Myogenic Potential

2:53

Transplantation in Mouse Models of Muscular Dystrophy

6:37

Outcome Measures on Transplanted Dystrophic Animals: Treadmill Test

8:15

Results: In vitro and In vivo Differentiation and Motor Capacity after Transplantation

9:24

Conclusion

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