JoVE Journal

Neuroscience

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Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

Chapters in this video

0:05

Title

2:05

General Set Up and Tissue Preparation

5:32

Patching Cell Bodies of Retinal Ganglion Cells

7:20

Recordings of Ganglion Cells Using Dynamic Clamp

9:12

Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells

10:14

Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

11:27

Conclusion

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