JoVE Journal

Immunology and Infection

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Methods Article

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

Chapters in this video

0:05

Title

1:27

Environmental Control Conditions for Live-cell Fluorescent Microscopy

3:44

Real-time Imaging of Anterograde Virion Transport Events

6:43

Overnight Time-lapse Imaging of Anterograde Spread Events

9:08

Image Processing and Export

10:48

Results: Representative Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

15:06

Conclusion

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