Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

15.1K Views

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11:22 min

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February 24th, 2014

DOI :

10.3791/50898-v

February 24th, 2014

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Ki Ho Park¹, Noah Weisleder², Jingsong Zhou³, Kristyn Gumpper¹, Xinyu Zhou¹, Pu Duann⁴, Jianjie Ma¹, Pei-Hui Lin¹

¹Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ²Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ³Department of Molecular Biophysics and Physiology, Rush University Medical Center, ⁴Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

Summary

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Calcium Sparks

Skeletal Muscle Fibers

Ryanodine Receptor

Sarcoplasmic Reticulum

Ca2 Signaling

Osmotic Stress

Fluorescent Ca2 Indicators

Laser Scanning Confocal Microscopy

Muscle Dystrophy

Amyotrophic Lateral Sclerosis

Chapters in this video

0:05

Title

1:29

Setting Up Osmotic-stress Perfusion System and Solution Preparation

2:00

Preparing Intact Single Flexor Digitorum Brevis (FDB) Muscle Fibers from Mouse

5:12

Fluo-4 AM Dye Loading and Ca²⁺ Imaging (Sparks Measurement)

7:39

Data Analysis

8:14

Results: Ca²⁺ Sparks in Young, Aged, and Diseased Mice

10:28

Conclusion

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