Methods Article

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

14.8K Views

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08:35 min

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February 21st, 2014

DOI :

10.3791/51510-v

February 21st, 2014

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Christopher W. Coyne¹, Karan Patel¹, Johanna Heureaux¹, Jeanne Stachowiak³, Daniel A. Fletcher⁴^,⁵, Allen P. Liu¹^,²

¹Department of Mechanical Engineering, University of Michigan, ²Department of Biomedical Engineering, University of Michigan, ³Department of Biomedical Engineering, Institute for Cellular and Molecular Biology, The University of Texas at Austin, ⁴Department of Bioengineering, University of California, Berkeley, ⁵Physical Biosciences Division, Lawrence Berkeley National Laboratory

Summary

Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.

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