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Biology

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Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

12.3K Views

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08:27 min

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July 24th, 2014

DOI :

10.3791/51519-v

July 24th, 2014

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Kevin G. Chen¹, Rebecca S. Hamilton¹, Pamela G. Robey², Barbara S. Mallon¹

¹NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, ²Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health

Summary

Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.

Explore More Videos

Human Pluripotent Stem Cells

Non colony Type Monolayer

Extracellular Matrix

Genetic Modification

Regenerative Medicine

Biopharmaceutical Applications

Chapters in this video

0:05

Title

1:15

Converting Human Pluripotent Stem Cell (hPSC) Colonies on Feeders to Noncolony Type Monolayers (NCM)

4:41

NCM Culture for Transfection of MicroRNAs

5:51

Results: Representative NCM Culture Analyses

7:48

Conclusion

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