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Methods Article
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
12.8K Views
•
13:05 min
•
June 13th, 2014
DOI :
June 13th, 2014
•Chapters in this video
0:05
Title
1:32
Transient Expression of TrCel5A and TrCel5A-mCherry Proteins
3:17
TrCel5A Extraction from Tobacco Leaves
4:42
SDS-PAGE, Western Blotting, and Azo-CMC Zymography of TrCel5A-mCherry
6:39
4-MUC Activity Assay of TrCel5A
7:50
Azo-carboxymethyl Cellulose Activity Assay of TrCel5A
9:01
PAHBAH Assay of TrCel5A
10:40
Results: Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
12:24
Conclusion
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