JoVE Journal

Immunology and Infection

A subscription to JoVE is required to view this content.

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

12.5K Views

•

11:10 min

•

August 11th, 2014

DOI :

10.3791/51759-v

August 11th, 2014

•

Jing Wu¹, Roberta Pugh¹, Richard C. Laughlin¹, Helene Andrews-Polymenis², Michael McClelland³, Andreas J. Bäumler⁴, L. Garry Adams¹

¹Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, ²Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M University System Health Science Center, ³University of California, Irvine, ⁴Department of Medical Microbiology & Immunology, School of Medicine, University of California, Davis

Summary

A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.

Explore More Videos

High throughput

Gentamicin Protection Assay

Deletion Mutants

Chapters in this video

0:05

Title

1:59

Murine Macrophage RAW264.7 Cell Culture

3:29

Preparation of Salmonella Wild-type and Mutants

5:19

Invasion Assay in a 96-well Culture Plate

9:58

Results: Identification of the Phenotype of Salmonella Mutants

10:46

Conclusion

Related Videos

article

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

21.6K Views

article

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

14.9K Views

article

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

11.5K Views

article

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

10.8K Views

article

High-throughput Gene Tagging in Trypanosoma brucei

7.9K Views

article

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

10.2K Views

article

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

7.7K Views

article

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

9.2K Views

article

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

10.5K Views

article

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

8.8K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More