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Immunology and Infection

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Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

5.4K Views

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09:18 min

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May 27th, 2015

DOI :

10.3791/52497-v

May 27th, 2015

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P. David McMullen¹, Nancy E. Freitag¹

¹Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago

Summary

Listeria monocytogenes causes fetal infections in pregnant women and meningitis in susceptible populations. Subpopulations of bacteria can colonize cardiac tissue, causing myocarditis in patients and laboratory animals. Here we present a protocol that describes how to assess L. monocytogenes cardiac cell invasion in vitro and cardiac colonization in infected animals.

Explore More Videos

Listeria Monocytogenes

Heart Colonization

Invasive Infection

Cardiac Pathology

Chapters in this video

0:05

Title

1:54

Invasion Assay

4:09

Inoculating L. monocytogenes into Mice via Tail Vein Injection and Assessing Bacterial Burden in Liver, Spleen, and Heart

7:39

Results: L. monocytogenes Infection in Cultured Organ Cells

8:47

Conclusion

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