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Developmental Biology

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Methods Article

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

18.5K Views

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11:53 min

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March 18th, 2015

DOI :

10.3791/52628-v

March 18th, 2015

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Arun Sharma*¹, Guang Li*¹, Kuppusamy Rajarajan*¹, Ryoko Hamaguchi¹, Paul W. Burridge¹, Sean M. Wu¹^,²

¹Stanford Cardiovascular Institute, Stanford University School of Medicine, ²Institute of Stem Cell Biology and Regenerative Medicine, Cardiovascular Medicine Division, Department of Medicine, Child Health Research Institute, Stanford University School of Medicine

Summary

Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.

Explore More Videos

Human Induced Pluripotent Stem Cells

Cardiomyocyte Differentiation

Small Molecule based Protocol

Wnt Catenin Signaling

Glucose Starvation

Purified Cardiomyocytes

In Vitro Disease Modeling

Chapters in this video

0:05

Title

1:56

Passaging of hiPSCs

4:52

Cardiac Differentiation of Human iPSC

7:17

Purification of Human Cardiomyocytes through Glucose Starvation

9:39

Representative Analysis of Differentiated Cardiomyocytes

10:55

Conclusion

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