JoVE Journal
Neuroscience
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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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11:24 min
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June 17th, 2015
DOI :
June 17th, 2015
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Chapters in this video
0:05
Title
1:26
Heat-Induced Antigen/RNA Unmasking
2:21
Probe Hybridization
4:01
Immunofluorescence Staining
5:42
Diaminobenzidine (DAB)-based Chromogenic ISH Assay Followed by Luxol Fast Blue and Cresyl Violet Stain Using Heat-induced and Protease-induced RNA Unmasking
8:54
Results: Aft4 Localization to Cholinergic Axons in the Mouse Dentate Gyrus and Myelinated Axons in the Adult Hippocampal Formation
10:53
Conclusion
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ISSN 1940-087X
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