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Neuroscience

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Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

9.3K Views

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09:10 min

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May 12th, 2015

DOI :

10.3791/52810-v

May 12th, 2015

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Magalie Bénard¹, Alexis Lebon¹, Hitoshi Komuro², David Vaudry¹, Ludovic Galas¹

¹PRIMACEN, Cell Imaging Platform of Normandy, Inserm, IRIB, University of Rouen, ²Department of Neurobiology, School of Medicine, Yale University

Summary

During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.

Explore More Videos

Cerebellar Granule Cell

Postnatal Development

Tangential Migration

Radial Migration

Cerebellar Cortex

Confocal Macroscopy

Real time Monitoring

Protease Inhibitor

Intracellular Effector

Chapters in this video

0:05

Title

1:22

Dissecting Cerebellums from P10 Rats

2:26

Preparing Acute Cerebellar Slices

4:27

Fluorescent Staining of Living Interneurons

5:44

Ex Vivo Imaging

6:27

Cell Tracking Using ImageJ

7:25

Results: Analysis of Granule Cell Migration

8:26

Conclusion

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