JoVE Journal

Developmental Biology

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Methods Article

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

Chapters in this video

0:05

Title

1:32

Cell Encapsulation into Alginate Hydrogel Capsules and Treatment of Alginate Capsules

3:44

Culture and Collect Cells in Alginate Capsules

4:56

Results: Visualizations of Alginate Capsule Formation and Morphologies of Mouse iPS Cells in Capsules

6:43

Conclusion

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