JoVE Journal

Behavior

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Electrophysiological Motor Unit Number Estimation (MUNE) Measuring Compound Muscle Action Potential (CMAP) in Mouse Hindlimb Muscles

20.9K Views

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09:07 min

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September 25th, 2015

DOI :

10.3791/52899-v

September 25th, 2015

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W. David Arnold¹^,²^,³, Kajri A. Sheth¹, Christopher G. Wier⁴, John T. Kissel¹^,³, Arthur H. Burghes¹^,³^,⁴, Stephen J. Kolb¹^,³^,⁴

¹Department of Neurology, The Ohio State University Wexner Medical Center, ²Department of Physical Medicine and Rehabilitation, The Ohio State University, ³Department of Neuroscience, The Ohio State University Wexner Medical Center, ⁴Department of Biochemistry and Pharmacology, The Ohio State University Wexner Medical Center

Summary

We present refined protocols that allow in vivo monitoring of motor unit function in the mouse. Techniques to measure compound muscle action potential (CMAP) and motor unit number estimation (MUNE) in the mouse hind limb muscles innervated by the sciatic nerve are described.

Explore More Videos

Electrophysiology

Motor Unit Number Estimation

Compound Muscle Action Potential

Mouse Hindlimb Muscles

Neuromuscular System

Motor Unit Potential

Submaximal Stimuli

Translational Potential

Chapters in this video

0:05

Title

2:17

Recording Setup and Equipment

3:46

Sciatic CMAP

4:44

Average Single Motor Unit Potential Size and MUNE Calculation

7:29

Results: Example Sciatic CMAP and MUNE

8:21

Conclusion

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