JoVE Journal

Immunology and Infection

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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of skin.

Chapters in this video

0:05

Title

1:16

Preparation of Instruments and Culture Medium

2:28

Preparation of Epidermal Sheets from Murine Newborn Skin or from Adult Tail Skin

4:31

Dissociation of Epidermis for FACS Analysis

5:57

Infection of Epidermal Sheets with Herpes Simplex Virus Type 1 and Visualization of Infected Cells

9:36

Results: Herpes Simplex Virus Infection of Murine Epidermal Sheets

11:20

Conclusion

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