JoVE Journal

Immunology and Infection

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Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.

Chapters in this video

0:05

Title

1:41

Preparation of Y. enterocolitica Strains for Cell Culture Infection

3:38

Infection of HeLa Cells and Loading with CCF4

5:44

Laser Scanning Microscopy

7:17

Image Quantification

10:52

Results: Analysis of Yersinia enterocolitica Effector Translocation

11:54

Conclusion

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