JoVE Journal

Environment

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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.

Chapters in this video

0:05

Title

2:11

High-throughput Determination of Glucose and Xylose Yields Following Enzymatic Saccharification

7:38

High-throughput Determination of Total Lignin and Lignin Monomeric Content Using pyMBMS

8:34

High-throughput Pretreatment, Enzyme Saccharification, and pyMBMS Biomass Samples

10:13

Conclusion

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