JoVE Journal

Environment

A subscription to JoVE is required to view this content.

live

MEDIA_ELEMENT_ERROR: Format error

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

7.0K Views

•

09:35 min

•

September 13th, 2019

DOI :

10.3791/59903-v

September 13th, 2019

•

Karel Šimek¹, Dagmara Sirova¹

¹Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre CAS

Transcript

This approach is significant because it allows us to visualize and estimate carbon and energy flows from prokaryotes to higher trophic levels in microbial food webs at a very high resolution.This is the only technique currently available for determining which protist species consume bacteria in situ, and at the same time quantifying the rate of this process at a single-cell level.Since bacteria are the only significant microbes changing dissolved organic carbon to particulate organic carbon, the knowledge of the players involved and rates at which this happens forms the basis for understanding aquatic ecosystems in general.We highly recommend that researchers become familiar with their study system before they conduct any grazing experiments.This in involves light and epifluorescence of microscopy inspection of the samples, and the knowledge of main taxon present and their seasonal dynamics.Demonstrating the procedure will be Jitka Jezberov

Summary

Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.

Explore More Videos

Fluorescently Labeled Bacteria

Organic Carbon Flow

Aquatic Ecosystems

Microbial Food Webs

Predator prey Interactions

Trophic Interactions

Nutrient Transfer

Single cell Analysis

Epifluorescence

Aquatic Microbial Communities

Chapters in this video

0:04

Title

1:11

Sample Collection and Fixation

1:44

Sample Filtration and Enumeration of Bacterial Numbers on the Filters

2:45

Determining Protistan Abundance

3:28

Estimating Ciliate Grazing Rates

4:33

Results: Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

8:07

Conclusion

Related Videos

article

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

12.2K Views

article

Alternative Method of Removing Otoliths from Sturgeon

8.4K Views

article

Assessment of Labile Organic Carbon in Soil Using Sequential Fumigation Incubation Procedures

11.4K Views

article

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems

10.1K Views

article

A Flow-through Exposure System for Evaluating Suspended Sediments Effects on Aquatic Life

8.3K Views

article

Production and Measurement of Organic Particulate Matter in a Flow Tube Reactor

7.4K Views

article

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

18.3K Views

article

Characterization of Aquatic Biofilms with Flow Cytometry

8.9K Views

article

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

11.0K Views

article

Use of Principal Components for Scaling Up Topographic Models to Map Soil Redistribution and Soil Organic Carbon

10.1K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More