Our research centers around skeletal muscle, which is comprised of cells with distinctive metabolic and contractile properties, characterized as slow or fast twitch fibers. We delve into the factors contributing to these differences and explore their implications in the context of aging, exercise, and various diseases. Measuring cellular responses in different skeletal muscle fibers is often overlooked, or not possible due to the examination of muscle homogenates.
The MyDoBID technique allows us to repeatedly analyze fiber type protein differences that were previously impossible to measure. This protocol accurately and efficiently identifies type one and type two muscle fibers from freeze dried human skeletal muscle samples. This newly revised method provides a straightforward approach for researchers to investigate protein differences in skeletal muscle at the cellular level.
Notably, it facilitates the identification of type two X fibers, and these have historically been challenging to distinguish. The technique allows researchers to shed light on all fiber types, including the elusive type two X fiber. With the MyDoBID technique, numerous questions can now be answered.
For example, one can discover the properties of type two X muscle fibers, and determine if there's evidence indicating preferential atrophy of type two X muscle fibers during aging or disease. One can also observe how a specific exercise intervention impacts each fiber type.
