JoVE Journal

Chemistry

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Author Spotlight: Plant Primary Organs Profiling Using <sup>13</sup>C<sub>6</sub>&#45;Glucose Labeling and LC&#45;MS

Transcript

We aimed to devise a universal and descriptive method for identifying the organs responsible for producing cyclical metabolics in medicinal plants. This method integrates certain carbon six glucose labeling with LC-MS indices, offering a process and accessible way to determine where these important compounds are synthesized. Most recent developments focus on the metabolite dynamics, protein quantification, and their exploration of complex biological matrices.

Multi spectrometry analysis methods, nuclear magnetic lances, spectral copy, bioinformatics, and the data analysis tools can promote the development of chemical isotope labeling methods. The main challenge of this experiment is metric effects. The presence of other elements or compounds in the sample can interfere with the curate measurement of carbon isotopes, leading to metric effects that can skew results.

Our results open avenues for several new scientific inquirers, including mechanisms of metabolic synthesis, genetic regulation, impact of environment factors, cross organ transport, and lecture practices.

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