JoVE Journal

Developmental Biology

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Methods Article

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

Chapters in this video

0:05

Title

0:40

Creating the Scratch

2:51

Taking Initial Images of the Scratch

4:34

Analyzing Initial Scratch Images

6:07

Taking Final Images of the Scratch and Analyzing Final Scratch Images

6:52

Results: Migratory of Murine Lung MSCs is Altered After Damage with Cigarette Smoke Extract

8:02

Conclusion

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